› 5kb3

X-ray diffraction
1.4Å resolution

1.4 A resolution structure of Helicobacter Pylori MTAN in complexed with p-ClPh-DADMe-ImmA

Released:
DOI: 10.2210/pdb5kb3/pdb
PDB entry 5kb3 coloured by chain, front view.
PDB entry 5kb3 coloured by chain, side view.
PDB entry 5kb3 coloured by chain, top view.
Source organism: Helicobacter pylori J99
Primary publication:
Neutron structures of the Helicobacter pylori 5'-methylthioadenosine nucleosidase highlight proton sharing and protonation states.
Banco MT, Mishra V, Ostermann A, Schrader TE, Evans GB, Kovalevsky A, Ronning DR
Proc Natl Acad Sci U S A 113 13756-13761 (2016)
PMID: 27856757

Function and Biology Details

Reactions catalysed: 
6-amino-6-deoxyfutalosine + H(2)O = dehypoxanthine futalosine + adenine
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminodeoxyfutalosine nucleosidase Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 24.98 KDa
Source organism: Helicobacter pylori J99
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZMY2 (Residues: 2-230; Coverage: 100%)
Gene names: jhp_0082, mtn, mtnN
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand 4CT 1 x 4CT
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3221
Unit cell:
a: 81.207Å b: 81.207Å c: 67.254Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.14 0.139 0.17
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Immucillins in Infectious Diseases.
Evans et al. (2018)
4 more

2 mentions without citation

Neutron structures of the Helicobacter pylori 5'-methylthioadenosine nucleosidase highlight proton sharing and protonation states.
Banco et al. (2016)
1 more