› 5kh0

X-ray diffraction
2.8Å resolution

Crystal Structure of HydF from thermosipho melanesiensis in complex with a [4Fe-4S] cluster

Released:
DOI: 10.2210/pdb5kh0/pdb
PDB entry 5kh0 coloured by chain, front view.
PDB entry 5kh0 coloured by chain, side view.
PDB entry 5kh0 coloured by chain, top view.
Source organism: Thermosipho melanesiensis BI429
Primary publication:
Structural and functional characterization of the hydrogenase-maturation HydF protein.
Caserta G, Pecqueur L, Adamska-Venkatesh A, Papini C, Roy S, Artero V, Atta M, Reijerse E, Lubitz W, Fontecave M
Nat Chem Biol 13 779-784 (2017)
PMID: 28553946

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107972 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small GTP-binding protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 396 amino acids
Theoretical weight: 44.35 KDa
Source organism: Thermosipho melanesiensis BI429
Expression system: Escherichia coli
UniProt:
  • Canonical: A6LMQ7 (Residues: 1-396; Coverage: 100%)
Gene name: Tmel_1361
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SF4 4 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 72.01Å b: 173.48Å c: 89.32Å
α: 90° β: 110.01° γ: 90°
R-values:
R R work R free
0.234 0.233 0.262
Expression system: Escherichia coli

Quick links

Citations

3 review citations

From protein engineering to artificial enzymes - biological and biomimetic approaches towards sustainable hydrogen production.
Esmieu et al. (2018)
2 more

3 mentions without citation

Biosynthesis of the catalytic H-cluster of [FeFe] hydrogenase: the roles of the Fe-S maturase proteins HydE, HydF, and HydG.
Britt et al. (2020)
2 more