› 5kjs

X-ray diffraction
2.2Å resolution

Crystal Structure of Arabidopsis thaliana HCT

Released:
DOI: 10.2210/pdb5kjs/pdb
PDB entry 5kjs coloured by chain, front view.
PDB entry 5kjs coloured by chain, side view.
PDB entry 5kjs coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Dynamic Conformational States Dictate Selectivity toward the Native Substrate in a Substrate-Permissive Acyltransferase.
Levsh O, Chiang YC, Tung CF, Noel JP, Wang Y, Weng JK
Biochemistry 55 6314-6326 (2016)
PMID: 27805809

Function and Biology Details

Reaction catalysed: 
4-coumaroyl-CoA + shikimate = CoA + 4-coumaroylshikimate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shikimate O-hydroxycinnamoyltransferase Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 48.62 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9FI78 (Residues: 1-433; Coverage: 100%)
Gene names: At5g48930, HCT, HST, K19E20.4
Sequence domains: Transferase family
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3221
Unit cell:
a: 99.91Å b: 99.91Å c: 83.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.18 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Promiscuity, impersonation and accommodation: evolution of plant specialized metabolism.
Leong et al. (2017)
3 more

4 mentions without citation

Dynamic Conformational States Dictate Selectivity toward the Native Substrate in a Substrate-Permissive Acyltransferase.
Levsh et al. (2016)
3 more