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X-ray diffraction
2.2Å resolution

Crystal Structure of GH3.5 Acyl Acid Amido Synthetase from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb5kod/pdb
PDB entry 5kod coloured by chain, front view.
PDB entry 5kod coloured by chain, side view.
PDB entry 5kod coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Arabidopsis thaliana GH3.5 acyl acid amido synthetase mediates metabolic crosstalk in auxin and salicylic acid homeostasis.
Westfall CS, Sherp AM, Zubieta C, Alvarez S, Schraft E, Marcellin R, Ramirez L, Jez JM
Proc Natl Acad Sci U S A 113 13917-13922 (2016)
PMID: 27849615

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Indole-3-acetic acid-amido synthetase GH3.5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 612 amino acids
Theoretical weight: 69.36 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O81829 (Residues: 1-612; Coverage: 100%)
Gene names: At4g27260, GH3.5, M4I22.70
Sequence domains: GH3 auxin-responsive promoter

Ligands and Environments

3 bound ligands:
Ligand AMP 4 x AMP
Ligand IAC 4 x IAC
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 91.609Å b: 143.523Å c: 102.322Å
α: 90° β: 114.72° γ: 90°
R-values:
R R work R free
0.207 0.205 0.245
Expression system: Escherichia coli

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Citations

14 review citations

Evolution of Hormone Signaling Networks in Plant Defense.
Berens et al. (2017)
13 more

3 mentions without citation

Arabidopsis thaliana GH3.5 acyl acid amido synthetase mediates metabolic crosstalk in auxin and salicylic acid homeostasis.
Westfall et al. (2016)
2 more