› 5kte

X-ray diffraction
3.94Å resolution

Crystal structure of Deinococcus radiodurans MntH, an Nramp-family transition metal transporter

Released:
DOI: 10.2210/pdb5kte/pdb
PDB entry 5kte coloured by chain, front view.
PDB entry 5kte coloured by chain, side view.
PDB entry 5kte coloured by chain, top view.
Source organisms:
Primary publication:
Crystal Structure and Conformational Change Mechanism of a Bacterial Nramp-Family Divalent Metal Transporter.
Bozzi AT, Bane LB, Weihofen WA, Singharoy A, Guillen ER, Ploegh HL, Schulten K, Gaudet R
Structure 24 2102-2114 (2016)
PMID: 27839948

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231410 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Divalent metal cation transporter MntH Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 45.26 KDa
Source organism: Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RTP8 (Residues: 26-436; Coverage: 94%)
Gene names: DR_1709, mntH
Sequence domains: Natural resistance-associated macrophage protein-like
Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 213 amino acids
Theoretical weight: 22.57 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand OS 3 x OS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I222
Unit cell:
a: 113.131Å b: 132.077Å c: 220.995Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.272 0.268 0.313
Expression systems:
  • Escherichia coli
  • Mus musculus

Quick links

Citations

2 review citations

Heteromeric Solute Carriers: Function, Structure, Pathology and Pharmacology.
Fairweather et al. (2021)
1 more

9 mentions without citation

Molecular Mechanism of Nramp-Family Transition Metal Transport.
Bozzi et al. (2021)
8 more