› 5kzw

X-ray diffraction
2Å resolution

Crystal structure of human GAA

Released:
DOI: 10.2210/pdb5kzw/pdb
PDB entry 5kzw coloured by chain, front view.
PDB entry 5kzw coloured by chain, side view.
PDB entry 5kzw coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Deming DT, Garman SC

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145218 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Lysosomal alpha-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 874 amino acids
Theoretical weight: 97.14 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P10253 (Residues: 79-952; Coverage: 95%)
Gene name: GAA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FCA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FCA
Carbohydrate polymer
7 bound ligands:
Ligand NAG 1 x NAG
Ligand GLC 2 x GLC
Ligand BGC 2 x BGC
Ligand EDO 3 x EDO
Ligand PGE 1 x PGE
Ligand PEG 2 x PEG
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P212121
Unit cell:
a: 97.11Å b: 102.59Å c: 128.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.185
Expression system: Cricetulus griseus

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