› 5lcm

X-ray diffraction
1.9Å resolution

STRUCTURE OF the RAD14 DNA-binding domain IN COMPLEX WITH N2-acetylaminonaphtyl- GUANINE CONTAINING DNA

Released:
DOI: 10.2210/pdb5lcm/pdb
PDB entry 5lcm coloured by chain, front view.
PDB entry 5lcm coloured by chain, side view.
PDB entry 5lcm coloured by chain, top view.
Source organisms:
Primary publication:
Structural Insights into the Recognition of N2 -Aryl- and C8-Aryl DNA Lesions by the Repair Protein XPA/Rad14.
Ebert C, Simon N, Schneider S, Carell T
Chembiochem 18 1379-1382 (2017)
PMID: 28444956

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118983 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA repair protein RAD14 Chains: A, B
Molecule details ›
Chains: A, B
Length: 119 amino acids
Theoretical weight: 14.22 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P28519 (Residues: 188-306; Coverage: 32%)
Gene names: RAD14, YM8325.02C, YMR201C
Sequence domains: XPA protein C-terminus
DNA (5'-D(*GP*CP*TP*CP*TP*AP*CP*(AAN)P*TP*CP*AP*TP*CP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
DNA (5'-D(*GP*TP*GP*AP*TP*GP*AP*CP*GP*TP*AP*GP*AP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P41
Unit cell:
a: 53.041Å b: 53.041Å c: 131.345Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.231 0.269
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

2 review citations

Non-Canonical Helical Structure of Nucleic Acids Containing Base-Modified Nucleotides.
Balasubramaniyam et al. (2021)
1 more