› 5ldh

X-ray diffraction
2.7Å resolution

STRUCTURE OF THE ACTIVE TERNARY COMPLEX OF PIG HEART LACTATE DEHYDROGENASE WITH S-LAC-NAD AT 2.7 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb5ldh/pdb
PDB entry 5ldh coloured by chain, front view.
PDB entry 5ldh coloured by chain, side view.
PDB entry 5ldh coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structure of the active ternary complex of pig heart lactate dehydrogenase with S-lac-NAD at 2.7 A resolution.
Grau UM, Trommer WE, Rossmann MG
J Mol Biol 151 289-307 (1981)
PMID: 7338899

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase B chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 36.51 KDa
Source organism: Sus scrofa
Expression system: Not provided
UniProt:
  • Canonical: P00336 (Residues: 2-334; Coverage: 100%)
Gene name: LDHB
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand LNC 2 x LNC
1 bound ligand:
Ligand CIT 2 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 133.6Å b: 133.6Å c: 113.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.196 not available
Expression system: Not provided

Quick links

Citations

7 review citations

DNA topoisomerases: structure, function, and mechanism.
Champoux JJ. (2001)
6 more

10 mentions without citation

Supramolecular chemistry in lipid bilayer membranes.
Bickerton et al. (2021)
9 more