› 5lhw

X-ray diffraction
0.91Å resolution

Central Coiled-Coil Domain of Human STIL

Released:
DOI: 10.2210/pdb5lhw/pdb
PDB entry 5lhw coloured by chain, front view.
PDB entry 5lhw coloured by chain, side view.
PDB entry 5lhw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A key centriole assembly interaction interface between human PLK4 and STIL appears to not be conserved in flies.
Cottee MA, Johnson S, Raff JW, Lea SM
Biol Open 6 381-389 (2017)
PMID: 28202467

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172301 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SCL-interrupting locus protein Chain: A
Molecule details ›
Chain: A
Length: 28 amino acids
Theoretical weight: 3.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli B
UniProt:
  • Canonical: Q15468 (Residues: 726-750; Coverage: 2%)
Gene names: SIL, STIL

Ligands and Environments

1 bound ligand:
Ligand P6G 1 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P42212
Unit cell:
a: 40.31Å b: 40.31Å c: 29.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.135 0.134 0.139
Expression system: Escherichia coli B

Quick links

Citations

8 citation in other articles

Two-step phosphorylation of Ana2 by Plk4 is required for the sequential loading of Ana2 and Sas6 to initiate procentriole formation.
Dzhindzhev et al. (2017)
7 more

1 mention without citation

Molecular Mechanism of STIL Coiled-Coil Domain Oligomerization.
Shamir et al. (2023)