› 5lil

X-ray diffraction
3.35Å resolution

Structure of Aggregatibacter actinomycetemcomitans MacB bound to ATPyS (P21)

Released:
DOI: 10.2210/pdb5lil/pdb
PDB entry 5lil coloured by chain, front view.
PDB entry 5lil coloured by chain, side view.
PDB entry 5lil coloured by chain, top view.
Source organism: Aggregatibacter actinomycetemcomitans
Primary publication:
Structure and mechanotransmission mechanism of the MacB ABC transporter superfamily.
Crow A, Greene NP, Kaplan E, Koronakis V
Proc Natl Acad Sci U S A 114 12572-12577 (2017)
PMID: 29109272

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrolide export ATP-binding/permease protein MacB Chains: A, B
Molecule details ›
Chains: A, B
Length: 664 amino acids
Theoretical weight: 72.11 KDa
Source organism: Aggregatibacter actinomycetemcomitans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2EHL8 (Residues: 1-644; Coverage: 100%)
Gene name: macB
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 111.587Å b: 82.621Å c: 124.92Å
α: 90° β: 94.45° γ: 90°
R-values:
R R work R free
0.247 0.244 0.29
Expression system: Escherichia coli BL21(DE3)

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Citations

12 review citations

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
11 more

13 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
12 more