› 5lka

X-ray diffraction
1.3Å resolution

Crystal structure of haloalkane dehalogenase LinB 140A+143L+177W+211L mutant (LinB86) from Sphingobium japonicum UT26 at 1.3 A resolution

Released:
DOI: 10.2210/pdb5lka/pdb
PDB entry 5lka coloured by chain, front view.
PDB entry 5lka coloured by chain, side view.
PDB entry 5lka coloured by chain, top view.
Source organism: Sphingobium indicum
Primary publication:
Engineering a de novo transport tunnel.
Brezovsky J, Babkova P, Degtjarik O, Fortova A, Gora A, Iermak I, Rezacova P, Dvorak P, Kuta Smatanova I, Prokop Z, Chaloupkova R, Damborsky J
ACS Catalysis (2016)

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 33.77 KDa
Source organism: Sphingobium indicum
Expression system: Escherichia coli
UniProt:
  • Canonical: D4Z2G1 (Residues: 2-296; Coverage: 100%)
Gene names: SJA_C1-19590, linB
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand SCN 1 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 46.867Å b: 68.362Å c: 80.648Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.138 0.174
Expression system: Escherichia coli

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