› 5lso

X-ray diffraction
2.22Å resolution

Crystal structure of SPF45 UHM domain with cyclic peptide inhibitor

Released:
DOI: 10.2210/pdb5lso/pdb
PDB entry 5lso coloured by chain, front view.
PDB entry 5lso coloured by chain, side view.
PDB entry 5lso coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Rational Design of Cyclic Peptide Inhibitors of U2AF Homology Motif (UHM) Domains To Modulate Pre-mRNA Splicing.
Jagtap PK, Garg D, Kapp TG, Will CL, Demmer O, Lührmann R, Kessler H, Sattler M
J Med Chem 59 10190-10197 (2016)
PMID: 27753493

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165962 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Splicing factor 45 Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 11.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96I25 (Residues: 301-400; Coverage: 25%)
Gene names: RBM17, SPF45
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
LYS-SER-ARG-TRP-ASP-GLU Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 amino acids
Theoretical weight: 822 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 37.6Å b: 73.59Å c: 45.01Å
α: 90° β: 97.9° γ: 90°
R-values:
R R work R free
0.197 0.181 0.244
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Roles and mechanisms of alternative splicing in cancer - implications for care.
Bonnal et al. (2020)
5 more

1 mention without citation

Bent Into Shape: Folded Peptides to Mimic Protein Structure and Modulate Protein Function.
Merritt et al. (2020)