› 5lv9

X-ray diffraction
2.33Å resolution

Crystal structure of thermophilic tryptophan halogenase (Th-Hal) enzyme from Streptomycin violaceusniger.

Released:
DOI: 10.2210/pdb5lv9/pdb
PDB entry 5lv9 coloured by chain, front view.
PDB entry 5lv9 coloured by chain, side view.
PDB entry 5lv9 coloured by chain, top view.
Source organism: Streptomyces violaceusniger
Primary publication:
Structure and biocatalytic scope of thermophilic flavin-dependent halogenase and flavin reductase enzymes.
Menon BR, Latham J, Dunstan MS, Brandenburger E, Klemstein U, Leys D, Karthikeyan C, Greaney MF, Shepherd SA, Micklefield J
Org Biomol Chem 14 9354-9361 (2016)
PMID: 27714222

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103535 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermophilic tryptophan halogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 513 amino acids
Theoretical weight: 57.97 KDa
Source organism: Streptomyces violaceusniger
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A1L1QK36 (Residues: 1-513; Coverage: 100%)
Sequence domains: Tryptophan halogenase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P65
Unit cell:
a: 67.14Å b: 67.14Å c: 477.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.224
Expression system: Escherichia coli K-12

Quick links

Citations

8 review citations

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.
Agarwal et al. (2017)
7 more

4 mentions without citation

Halogenases: a palette of emerging opportunities for synthetic biology-synthetic chemistry and C-H functionalisation.
Crowe et al. (2021)
3 more