› 5lxy

X-ray diffraction
2.85Å resolution

Structure of the minimal RBM7 - ZCCHC8 Complex

Released:
DOI: 10.2210/pdb5lxy/pdb
PDB entry 5lxy coloured by chain, front view.
PDB entry 5lxy coloured by chain, side view.
PDB entry 5lxy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors.
Falk S, Finogenova K, Melko M, Benda C, Lykke-Andersen S, Jensen TH, Conti E
Nat Commun 7 13573 (2016)
PMID: 27905398

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180092 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-binding protein 7 Chains: A, B, E, G, I, K, M
Molecule details ›
Chains: A, B, E, G, I, K, M
Length: 90 amino acids
Theoretical weight: 10.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y580 (Residues: 1-86; Coverage: 32%)
Gene name: RBM7
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Zinc finger CCHC domain-containing protein 8 Chains: C, D, F, H, J, L, N
Molecule details ›
Chains: C, D, F, H, J, L, N
Length: 45 amino acids
Theoretical weight: 5.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6NZY4 (Residues: 285-324; Coverage: 6%)
Gene name: ZCCHC8
Sequence domains: PSP

Ligands and Environments

1 bound ligand:
Ligand BR 9 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 178.771Å b: 66.575Å c: 111.912Å
α: 90° β: 126.57° γ: 90°
R-values:
R R work R free
0.262 0.262 0.3
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Targeting RNA for processing or destruction by the eukaryotic RNA exosome and its cofactors.
Zinder et al. (2017)
5 more

3 mentions without citation

Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors.
Falk et al. (2016)
2 more