› 5ly8

X-ray diffraction
1.28Å resolution

Structure of the CBM2 module of Lactobacillus casei BL23 phage J-1 evolved Dit.

Released:
DOI: 10.2210/pdb5ly8/pdb
PDB entry 5ly8 coloured by chain, front view.
PDB entry 5ly8 coloured by chain, side view.
PDB entry 5ly8 coloured by chain, top view.
Source organism: Lactobacillus phage J-1
Primary publication:
Evolved distal tail carbohydrate binding modules of Lactobacillus phage J-1: a novel type of anti-receptor widespread among lactic acid bacteria phages.
Dieterle ME, Spinelli S, Sadovskaya I, Piuri M, Cambillau C
Mol Microbiol 104 608-620 (2017)
PMID: 28196397

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Siphovirus-type tail component RIFT-related domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 247 amino acids
Theoretical weight: 27.73 KDa
Source organism: Lactobacillus phage J-1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: U5U3S0 (Residues: 368-614; Coverage: 36%)
Gene name: J1_16

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 49.02Å b: 63.67Å c: 74.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 0.198
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

In Vitro Characteristics of Phages to Guide 'Real Life' Phage Therapy Suitability.
Casey et al. (2018)
3 more

6 mentions without citation

Molecular Basis of Bacterial Host Interactions by Gram-Positive Targeting Bacteriophages.
Dunne et al. (2018)
5 more