Function and Biology Details
Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
ATP + H(2)O = ADP + phosphate
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131639 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Golgi resident protein GCP60, N-terminally processed
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 19.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: Golgi-dynamics membrane-trafficking
Length: 166 amino acids
Theoretical weight: 19.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
- Canonical:
Q9H3P7 (Residues: 364-528; Coverage: 31%)
Sequence domains: Golgi-dynamics membrane-trafficking
Protein 3A
Molecule details ›
Chain: B
Length: 58 amino acids
Theoretical weight: 6.61 KDa
Source organism: Aichivirus A
Expression system: Escherichia coli BL21
UniProt:
Length: 58 amino acids
Theoretical weight: 6.61 KDa
Source organism: Aichivirus A
Expression system: Escherichia coli BL21
UniProt:
- Canonical: O91464 (Residues: 1653-1710; Coverage: 2%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
RIGAKU MICROMAX-007 HF
Spacegroup:
I222
Expression system: Escherichia coli BL21
