› 5m5i

Electron Microscopy
9.3Å resolution

Pseudo-atomic model of microtubule-bound S.pombe kinesin-5 motor domain in the AMPPNP state (based on cryo-electron microscopy experiment): the N-terminus conformation allows formation of a cover neck bundle.

Released:
DOI: 10.2210/pdb5m5i/pdb
PDB entry 5m5i coloured by chain, front view.
PDB entry 5m5i coloured by chain, side view.
PDB entry 5m5i coloured by chain, top view.
Source organisms:
Primary publication:
Schizosaccharomyces pombe kinesin-5 switches direction using a steric blocking mechanism.
Britto M, Goulet A, Rizvi S, von Loeffelholz O, Moores CA, Cross RA
Proc Natl Acad Sci U S A 113 E7483-E7489 (2016)
PMID: 27834216
Related structures: EMD-3445

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-150213 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1D chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.11 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2HJ86 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1D
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Kinesin-like protein cut7 Chain: C
Molecule details ›
Chain: C
Length: 369 amino acids
Theoretical weight: 40.74 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24339 (Residues: 64-432; Coverage: 34%)
Gene names: SPAC25G10.07c, cut7
Sequence domains: Kinesin motor domain

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand GTP 1 x GTP
Ligand GDP 1 x GDP
Ligand TA1 1 x TA1
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.3Å
Relevant EMDB volumes: EMD-3445
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Mitotic spindle: kinetochore fibers hold on tight to interpolar bundles.
Tolić IM. (2018)
3 more

3 mentions without citation

Schizosaccharomyces pombe kinesin-5 switches direction using a steric blocking mechanism.
Britto et al. (2016)
2 more