› 5m73

X-ray diffraction
3.4Å resolution

Structure of the human SRP S domain with SRP72 RNA-binding domain

Released:
DOI: 10.2210/pdb5m73/pdb
PDB entry 5m73 coloured by chain, front view.
PDB entry 5m73 coloured by chain, side view.
PDB entry 5m73 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of human SRP72 complexes provide insights into SRP RNA remodeling and ribosome interaction.
Becker MM, Lapouge K, Segnitz B, Wild K, Sinning I
Nucleic Acids Res 45 470-481 (2017)
PMID: 27899666

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-131215 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Signal recognition particle 19 kDa protein Chains: B, F
Molecule details ›
Chains: B, F
Length: 128 amino acids
Theoretical weight: 14.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09132 (Residues: 11-118; Coverage: 75%)
Gene name: SRP19
Sequence domains: SRP19 protein
Structure domains: Signal recognition particle, SRP19-like subunit
Signal recognition particle subunit SRP68 Chains: C, G
Molecule details ›
Chains: C, G
Length: 203 amino acids
Theoretical weight: 24.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHB9 (Residues: 60-254; Coverage: 31%)
Gene name: SRP68
Sequence domains: RNA-binding signal recognition particle 68
Structure domains: Signal recognition particle, SRP68 subunit, RNA-binding domain
Signal recognition particle subunit SRP72 Chains: D, H
Molecule details ›
Chains: D, H
Length: 158 amino acids
Theoretical weight: 17.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O76094 (Residues: 512-668; Coverage: 23%)
Gene name: SRP72
Sequence domains: SRP72 RNA-binding domain
Human gene for small cytoplasmic 7SL RNA (7L30.1) Chains: A, E
Molecule details ›
Chains: A, E
Length: 145 nucleotides
Theoretical weight: 47.2 KDa
Sequence domains: Metazoan signal recognition particle RNA

Ligands and Environments

3 bound ligands:
Ligand MG 45 x MG
Ligand K 10 x K
Ligand GOL 9 x GOL
1 modified residue:
Ligand GTP 2 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 134.567Å b: 139.306Å c: 152.246Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.237 0.28
Expression system: Escherichia coli

Quick links

Citations

5 review citations

SRPassing Co-translational Targeting: The Role of the Signal Recognition Particle in Protein Targeting and mRNA Protection.
Kellogg et al. (2021)
4 more

3 mentions without citation

Structures of human SRP72 complexes provide insights into SRP RNA remodeling and ribosome interaction.
Becker et al. (2017)
2 more