› 5m7x

X-ray diffraction
1.68Å resolution

Translation initiation factor 4E in complex with (RP)-m2(7,2'O)GppSepG mRNA 5' cap analog (beta-Se-ARCA D1)

Released:
DOI: 10.2210/pdb5m7x/pdb
PDB entry 5m7x coloured by chain, front view.
PDB entry 5m7x coloured by chain, side view.
PDB entry 5m7x coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Warminski M, Nowak E, Kowalska J, Jemielity J, Nowotny M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 4E Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 190 amino acids
Theoretical weight: 22.15 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63073 (Residues: 28-217; Coverage: 88%)
Gene name: Eif4e
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e

Ligands and Environments

2 bound ligands:
Ligand RSE 4 x RSE
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P1
Unit cell:
a: 38.11Å b: 38.16Å c: 147.07Å
α: 88.55° β: 95.32° γ: 103.33°
R-values:
R R work R free
0.192 0.192 0.219
Expression system: Escherichia coli

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