› 5mle

X-ray diffraction
2.48Å resolution

Crystal Structure of Human Dihydropyrimidinease-like 2 (DPYSL2A)/Collapsin Response Mediator Protein (CRMP2 13-516) Mutant Y479E/Y499E

Released:
DOI: 10.2210/pdb5mle/pdb
PDB entry 5mle coloured by chain, front view.
PDB entry 5mle coloured by chain, side view.
PDB entry 5mle coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tuning microtubule dynamics to enhance cancer therapy by modulating FER-mediated CRMP2 phosphorylation.
Zheng Y, Sethi R, Mangala LS, Taylor C, Goldsmith J, Wang M, Masuda K, Karaminejadranjbar M, Mannion D, Miranda F, Herrero-Gonzalez S, Hellner K, Chen F, Alsaadi A, Albukhari A, Fotso DC, Yau C, Jiang D, Pradeep S, Rodriguez-Aguayo C, Lopez-Berestein G, Knapp S, Gray NS, Campo L, Myers KA, Dhar S, Ferguson D, Bast RC, Sood AK, von Delft F, Ahmed AA
Nat Commun 9 476 (2018)
PMID: 29396402

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydropyrimidinase-related protein 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 504 amino acids
Theoretical weight: 55.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16555 (Residues: 13-516; Coverage: 88%)
Gene names: CRMP2, DPYSL2, ULIP2
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PEG 3 x PEG
Ligand EDO 10 x EDO
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2221
Unit cell:
a: 70.9Å b: 185.82Å c: 196.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.254
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Dysregulation of CRMP2 Post-Translational Modifications Drive Its Pathological Functions.
Moutal et al. (2019)
22 other articles

2 mentions without citation

Tuning microtubule dynamics to enhance cancer therapy by modulating FER-mediated CRMP2 phosphorylation.
Zheng et al. (2018)
1 more