› 5mom

X-ray diffraction
2.27Å resolution

Crystal Structure of PCNA encoding the hypomorphic mutation S228I

Released:
DOI: 10.2210/pdb5mom/pdb
PDB entry 5mom coloured by chain, front view.
PDB entry 5mom coloured by chain, side view.
PDB entry 5mom coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
PCNA dependent cellular activities tolerate dramatic perturbations in PCNA client interactions.
Wilson RH, Biasutto AJ, Wang L, Fischer R, Baple EL, Crosby AH, Mancini EJ, Green CM
DNA Repair (Amst) 50 22-35 (2017)
PMID: 28073635

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146041 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proliferating cell nuclear antigen Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 258 amino acids
Theoretical weight: 28.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12004 (Residues: 1-258; Coverage: 99%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P41212
Unit cell:
a: 162.95Å b: 162.95Å c: 140.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Link between the unfolded protein response and dysregulation of mitochondrial bioenergetics in Alzheimer's disease.
Poirier et al. (2019)
5 more

1 mention without citation

PCNA dependent cellular activities tolerate dramatic perturbations in PCNA client interactions.
Wilson et al. (2017)