› 5n2q

X-ray diffraction
2Å resolution

MobM Relaxase Domain (MOBV; Mob_Pre) bound to 26nt pMV158 oriT DNA

Released:
DOI: 10.2210/pdb5n2q/pdb
PDB entry 5n2q coloured by chain, front view.
PDB entry 5n2q coloured by chain, side view.
PDB entry 5n2q coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of a histidine-DNA nicking/joining mechanism for gene transfer and promiscuous spread of antibiotic resistance.
Pluta R, Boer DR, Lorenzo-Díaz F, Russi S, Gómez H, Fernández-López C, Pérez-Luque R, Orozco M, Espinosa M, Coll M
Proc Natl Acad Sci U S A 114 E6526-E6535 (2017)
PMID: 28739894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119043 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Plasmid recombination enzyme Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 23.59 KDa
Source organism: Streptococcus agalactiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P13925 (Residues: 2-198; Coverage: 40%)
Gene names: mob, pre
Sequence domains: Plasmid recombination enzyme
Structure domains: BirA Bifunctional Protein; domain 2
DNA (26-MER) Chain: B
Molecule details ›
Chain: B
Length: 27 nucleotides
Theoretical weight: 8.38 KDa
Source organism: Plasmid pMV158
Expression system: synthetic construct

Ligands and Environments

4 bound ligands:
Ligand CL 1 x CL
Ligand MG 1 x MG
Ligand GOL 1 x GOL
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 43.576Å b: 52.853Å c: 56.035Å
α: 90° β: 95.88° γ: 90°
R-values:
R R work R free
0.17 0.166 0.238
Expression systems:
  • Escherichia coli
  • synthetic construct

Quick links

Citations

1 review citation

Regulation of Gram-Positive Conjugation.
Kohler et al. (2019)
13 other articles

3 mentions without citation

The Facts and Family Secrets of Plasmids That Replicate via the Rolling-Circle Mechanism.
Garcillán-Barcia et al. (2022)
2 more