› 5n6u

X-ray diffraction
3.08Å resolution

Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum.

Released:
DOI: 10.2210/pdb5n6u/pdb
PDB entry 5n6u coloured by chain, front view.
PDB entry 5n6u coloured by chain, side view.
PDB entry 5n6u coloured by chain, top view.
Source organism: Dictyoglomus thermophilum H-6-12
Primary publication:
Is the acid/base catalytic residue mutation in β-d-mannosidase DtMan from Dictyoglomus thermophilum sufficient enough to provide thioglycoligase activity?
Guillotin L, Richet N, Lafite P, Daniellou R
Biochimie 137 190-196 (2017)
PMID: 28385558

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110271 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-mannosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 836 amino acids
Theoretical weight: 98.69 KDa
Source organism: Dictyoglomus thermophilum H-6-12
Expression system: Escherichia coli
UniProt:
  • Canonical: B5YAN4 (Residues: 1-813; Coverage: 100%)
Gene name: DICTH_1692
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BMA 4 x BMA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 139.25Å b: 75.56Å c: 217.88Å
α: 90° β: 92.52° γ: 90°
R-values:
R R work R free
0.206 0.204 0.255
Expression system: Escherichia coli

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Citations

1 review citation

Enzymatic synthesis of glycosides: from natural O- and N-glycosides to rare C- and S-glycosides.
Ati et al. (2017)
3 other articles