› 5ncj

X-ray diffraction
1.53Å resolution

GriE in complex with manganese, succinate and (2S,4R)-5-hydroxyleucine

Released:
DOI: 10.2210/pdb5ncj/pdb
PDB entry 5ncj coloured by chain, front view.
PDB entry 5ncj coloured by chain, side view.
PDB entry 5ncj coloured by chain, top view.
Source organism: Streptomyces muensis
Primary publication:
Biosynthesis of methyl-proline containing griselimycins, natural products with anti-tuberculosis activity.
Lukat P, Katsuyama Y, Wenzel S, Binz T, König C, Blankenfeldt W, Brönstrup M, Müller R
Chem Sci 8 7521-7527 (2017)
PMID: 29163906

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine hydroxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 268 amino acids
Theoretical weight: 29.86 KDa
Source organism: Streptomyces muensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0E3URV8 (Residues: 1-265; Coverage: 98%)
Gene name: griE
Sequence domains: Phytanoyl-CoA dioxygenase (PhyH)

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand SIN 2 x SIN
Ligand HL5 2 x HL5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 148.74Å b: 56.061Å c: 73.802Å
α: 90° β: 112.46° γ: 90°
R-values:
R R work R free
0.156 0.155 0.173
Expression system: Escherichia coli BL21(DE3)

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Citations

14 review citations

Amazing Diversity in Biochemical Roles of Fe(II)/2-Oxoglutarate Oxygenases.
Herr et al. (2018)
13 more

1 mention without citation

Biosynthesis of methyl-proline containing griselimycins, natural products with anti-tuberculosis activity.
Lukat et al. (2017)