› 5nd4

Electron Microscopy
4.4Å resolution

Microtubule-bound MKLP2 motor domain in the presence of ADP.AlFx

Released:
DOI: 10.2210/pdb5nd4/pdb
PDB entry 5nd4 coloured by chain, front view.
PDB entry 5nd4 coloured by chain, side view.
PDB entry 5nd4 coloured by chain, top view.
Source organisms:
Primary publication:
The divergent mitotic kinesin MKLP2 exhibits atypical structure and mechanochemistry.
Atherton J, Yu IM, Cook A, Muretta JM, Joseph A, Major J, Sourigues Y, Clause J, Topf M, Rosenfeld SS, Houdusse A, Moores CA
Elife 6 (2017)
PMID: 28826477
Related structures: EMD-3622

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-160562 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Kinesin-like protein KIF20A Chain: C
Molecule details ›
Chain: C
Length: 501 amino acids
Theoretical weight: 56.02 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P97329 (Residues: 21-521; Coverage: 57%)
Gene names: Kif20a, Rab6kifl
Sequence domains: Kinesin motor domain
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 412 amino acids
Theoretical weight: 45.96 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 2-439; Coverage: 91%)
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 426 amino acids
Theoretical weight: 47.81 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 2-427; Coverage: 96%)
Gene name: TUBB2B
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 2 x MG
Ligand ALF 1 x ALF
Ligand GTP 1 x GTP
Ligand GDP 1 x GDP
Ligand TA1 1 x TA1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-3622
Expression system: Escherichia coli

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Citations

5 review citations

How Kinesin-1 Utilize the Energy of Nucleotide: The Conformational Changes and Mechanochemical Coupling in the Unidirectional Motion of Kinesin-1.
Qin et al. (2020)
4 more

1 mention without citation

A microtubule RELION-based pipeline for cryo-EM image processing.
Cook et al. (2020)