› 5nhr

X-ray diffraction
3.35Å resolution

CRYSTAL STRUCTURE OF THE Activin receptor type-2B LIGAND BINDING DOMAIN IN COMPLEX WITH BIMAGRUMAB FV, CUBIC CRYSTAL FORM

Released:
DOI: 10.2210/pdb5nhr/pdb
PDB entry 5nhr coloured by chain, front view.
PDB entry 5nhr coloured by chain, side view.
PDB entry 5nhr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Blockade of activin type II receptors with a dual anti-ActRIIA/IIB antibody is critical to promote maximal skeletal muscle hypertrophy.
Morvan F, Rondeau JM, Zou C, Minetti G, Scheufler C, Scharenberg M, Jacobi C, Brebbia P, Ritter V, Toussaint G, Koelbing C, Leber X, Schilb A, Witte F, Lehmann S, Koch E, Geisse S, Glass DJ, Lach-Trifilieff E
Proc Natl Acad Sci U S A 114 12448-12453 (2017)
PMID: 29109273

Function and Biology Details

Reaction catalysed: 
ATP + [receptor-protein] = ADP + [receptor-protein] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212014 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Bimagrumab Fv Light-Chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 119 amino acids
Theoretical weight: 12.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli str. K-12 substr. W3110
Bimagrumab Fv heavy-chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 121 amino acids
Theoretical weight: 13.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli str. K-12 substr. W3110
Activin receptor type-2B Chains: C, D
Molecule details ›
Chains: C, D
Length: 99 amino acids
Theoretical weight: 11.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q13705 (Residues: 24-117; Coverage: 19%)
Gene name: ACVR2B
Sequence domains: Activin types I and II receptor domain
Structure domains: CD59

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P23
Unit cell:
a: 187.826Å b: 187.826Å c: 187.826Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.229
Expression systems:
  • Escherichia coli str. K-12 substr. W3110
  • Escherichia coli K-12

Quick links

Citations

22 review citations

Role of Myokines in Regulating Skeletal Muscle Mass and Function.
Lee et al. (2019)
21 more

1 mention without citation

Blockade of activin type II receptors with a dual anti-ActRIIA/IIB antibody is critical to promote maximal skeletal muscle hypertrophy.
Morvan et al. (2017)