› 5nqa

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-galactosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-alpha-D-galactosaminyl)-L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166032 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polypeptide N-acetylgalactosaminyltransferase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 578 amino acids
Theoretical weight: 66.76 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q8N4A0 (Residues: 1-578; Coverage: 100%)
Gene name: GALNT4
Sequence domains:
Monoglycopeptide 3 Chain: G
Molecule details ›
Chain: G
Length: 16 amino acids
Theoretical weight: 1.2 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand EDO 71 x EDO
Ligand GOL 10 x GOL
Ligand NGA 2 x NGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 65.447Å b: 79.881Å c: 88.614Å
α: 116.47° β: 96.74° γ: 104.72°
R-values:
R R work R free
0.226 0.225 0.274
Expression systems:
  • Komagataella pastoris
  • Not provided

Quick links

Citations

4 review citations

Emerging structural insights into glycosyltransferase-mediated synthesis of glycans.
Moremen et al. (2019)
3 more

6 mentions without citation

Polypeptide GalNAc-Ts: from redundancy to specificity.
de Las Rivas et al. (2019)
5 more