› 5o5b

Electron Microscopy
3.6Å resolution

Poliovirus type 3 (strain Saukett) stabilized virus-like particle

Released:
DOI: 10.2210/pdb5o5b/pdb
PDB entry 5o5b coloured by chain, front view.
PDB entry 5o5b coloured by chain, side view.
PDB entry 5o5b coloured by chain, top view.
Source organism: Poliovirus 3
Primary publication:
Plant-made polio type 3 stabilized VLPs-a candidate synthetic polio vaccine.
Marsian J, Fox H, Bahar MW, Kotecha A, Fry EE, Stuart DI, Macadam AJ, Rowlands DJ, Lomonossoff GP
Nat Commun 8 245 (2017)
PMID: 28811473
Related structures: EMD-3747

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 240-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136733 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Capsid proteins Chain: 1
Molecule details ›
Chain: 1
Length: 300 amino acids
Theoretical weight: 33.56 KDa
Source organism: Poliovirus 3
Expression system: Nicotiana benthamiana
UniProt:
  • Canonical: Q84895 (Residues: 579-878; Coverage: 34%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid proteins Chain: 2
Molecule details ›
Chain: 2
Length: 271 amino acids
Theoretical weight: 30.19 KDa
Source organism: Poliovirus 3
Expression system: Nicotiana benthamiana
UniProt:
  • Canonical: Q84895 (Residues: 70-340; Coverage: 31%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid proteins Chain: 3
Molecule details ›
Chain: 3
Length: 238 amino acids
Theoretical weight: 26.32 KDa
Source organism: Poliovirus 3
Expression system: Nicotiana benthamiana
UniProt:
  • Canonical: Q84895 (Residues: 341-578; Coverage: 27%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP4 Chain: 4
Molecule details ›
Chain: 4
Length: 69 amino acids
Theoretical weight: 7.45 KDa
Source organism: Poliovirus 3
Expression system: Nicotiana benthamiana
UniProt:
  • Canonical: P03302 (Residues: 1-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-3747
Expression system: Nicotiana benthamiana

Quick links

Citations

21 review citations

The life cycle of non-polio enteroviruses and how to target it.
Baggen et al. (2018)
20 more

4 mentions without citation

Plant-made polio type 3 stabilized VLPs-a candidate synthetic polio vaccine.
Marsian et al. (2017)
3 more