› 5o6i

X-ray diffraction
2.25Å resolution

Structures and dynamics of mesophilic variants from the homing endonuclease I-DmoI

Released:
DOI: 10.2210/pdb5o6i/pdb
PDB entry 5o6i coloured by chain, front view.
PDB entry 5o6i coloured by chain, side view.
PDB entry 5o6i coloured by chain, top view.
Source organisms:
Primary publication:
Structure and dynamics of mesophilic variants from the homing endonuclease I-DmoI.
Alba J, Marcaida MJ, Prieto J, Montoya G, Molina R, D'Abramo M
J Comput Aided Mol Des 31 1063-1072 (2017)
PMID: 29177929

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119086 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease I-DmoI Chains: A, F, K
Molecule details ›
Chains: A, F, K
Length: 200 amino acids
Theoretical weight: 23.42 KDa
Source organism: Desulfurococcus mucosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21505 (Residues: 2-188; Coverage: 96%)
Sequence domains: LAGLIDADG-like domain
Structure domains: Homing endonucleases
DNA (25-MER) Chains: C, G, L
Molecule details ›
Chains: C, G, L
Length: 25 nucleotides
Theoretical weight: 7.71 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (25-MER) Chains: D, I, N
Molecule details ›
Chains: D, I, N
Length: 25 nucleotides
Theoretical weight: 7.65 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MN 6 x MN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 107Å b: 70.48Å c: 107.32Å
α: 90° β: 119.81° γ: 90°
R-values:
R R work R free
0.198 0.196 0.228
Expression systems:
  • Escherichia coli
  • Not provided

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