› 5oed

X-ray diffraction
2.9Å resolution

Human Rab32:GDP in complex with Salmonella GtgE C45A mutant

Released:
DOI: 10.2210/pdb5oed/pdb
PDB entry 5oed coloured by chain, front view.
PDB entry 5oed coloured by chain, side view.
PDB entry 5oed coloured by chain, top view.
Source organisms:
Primary publication:
The protease GtgE from Salmonella exclusively targets inactive Rab GTPases.
Wachtel R, Bräuning B, Mader SL, Ecker F, Kaila VRI, Groll M, Itzen A
Nat Commun 9 44 (2018)
PMID: 29298974

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171804 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GtgE Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 26.36 KDa
Source organism: Salmonella enterica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6EAT3 (Residues: 1-228; Coverage: 100%)
Gene names: A7D45_11990, AL463_22290, AZF90_21570, CQW68_16290, D3Q81_09245, DT651_08660, EKD96_19760, F1527_14270, F2D26_09345, FJR52_13185, GCH85_24895, gtgE
Ras-related protein Rab-32 Chain: B
Molecule details ›
Chain: B
Length: 183 amino acids
Theoretical weight: 20.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13637 (Residues: 20-201; Coverage: 81%)
Gene name: RAB32
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GDP 1 x GDP
1 modified residue:
Ligand MLY 5 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6522
Unit cell:
a: 67.41Å b: 67.41Å c: 427.34Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.201 0.24
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Speaking the host language: how Salmonella effector proteins manipulate the host.
Pillay et al. (2023)
2 more

2 mentions without citation

The protease GtgE from Salmonella exclusively targets inactive Rab GTPases.
Wachtel et al. (2018)
1 more