› 5omd

X-ray diffraction
2.1Å resolution

Crystal structure of S. cerevisiae Ddc2 N-terminal coiled-coil domain

Released:
DOI: 10.2210/pdb5omd/pdb
PDB entry 5omd coloured by chain, front view.
PDB entry 5omd coloured by chain, side view.
PDB entry 5omd coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural Basis of Mec1-Ddc2-RPA Assembly and Activation on Single-Stranded DNA at Sites of Damage.
Deshpande I, Seeber A, Shimada K, Keusch JJ, Gut H, Gasser SM
Mol Cell 68 431-445.e5 (2017)
PMID: 29033322

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170023 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA damage checkpoint protein LCD1 Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.73 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04377 (Residues: 73-136; Coverage: 9%)
Gene names: DDC2, LCD1, PIE1, YDR499W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P2221
Unit cell:
a: 116.94Å b: 19.89Å c: 30.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.228 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Structural basis of homologous recombination.
Sun et al. (2020)
9 more

1 mention without citation

Novel insights into the mechanism of cell cycle kinases Mec1(ATR) and Tel1(ATM).
Tannous et al. (2021)