› 5suy

X-ray diffraction
1.88Å resolution

Domain-swapped dimer of human Dishevelled2 DEP domain: monoclinic crystal form crystallised from dimeric fraction

Released:
DOI: 10.2210/pdb5suy/pdb
PDB entry 5suy coloured by chain, front view.
PDB entry 5suy coloured by chain, side view.
PDB entry 5suy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Wnt Signalosome Assembly by DEP Domain Swapping of Dishevelled.
Gammons MV, Renko M, Johnson CM, Rutherford TJ, Bienz M
Mol Cell 64 92-104 (2016)
PMID: 27692984

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Segment polarity protein dishevelled homolog DVL-2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 97 amino acids
Theoretical weight: 10.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14641 (Residues: 416-510; Coverage: 13%)
Gene name: DVL2
Sequence domains: Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 61.671Å b: 60.174Å c: 66.128Å
α: 90° β: 117.62° γ: 90°
R-values:
R R work R free
0.186 0.185 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities.
Nusse et al. (2017)
29 more

2 mentions without citation

Wnt5a promotes Frizzled-4 signalosome assembly by stabilizing cysteine-rich domain dimerization.
DeBruine et al. (2017)
1 more