› 5swf

X-ray diffraction
2.82Å resolution

The structure of the PP2A B56 subunit double phosphorylated BubR1 complex

Released:
DOI: 10.2210/pdb5swf/pdb
PDB entry 5swf coloured by chain, front view.
PDB entry 5swf coloured by chain, side view.
PDB entry 5swf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Expanding the PP2A Interactome by Defining a B56-Specific SLiM.
Wang X, Bajaj R, Bollen M, Peti W, Page R
Structure 24 2174-2181 (2016)
PMID: 27998540

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130031 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 41.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13362 (Residues: 31-380; Coverage: 67%)
Gene names: KIAA0044, PPP2R5C
Sequence domains: Protein phosphatase 2A regulatory B subunit (B56 family)
Structure domains: Leucine-rich Repeat Variant
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.16 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O60566 (Residues: 668-676; Coverage: 1%)
Gene names: BUB1B, BUBR1, MAD3L, SSK1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 53.307Å b: 107.422Å c: 117.719Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.23
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

24 review citations

Kinase and Phosphatase Cross-Talk at the Kinetochore.
Saurin AT. (2018)
23 more

3 mentions without citation

Propolis, Bee Honey, and Their Components Protect against Coronavirus Disease 2019 (COVID-19): A Review of In Silico, In Vitro, and Clinical Studies.
Ali et al. (2021)
2 more