› 5t61

X-ray diffraction
2.55Å resolution

TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE FROM METHANOTHERMOBACTER WOLFEII, TRICLINIC FORM AT 2.55 A

Released:
DOI: 10.2210/pdb5t61/pdb
PDB entry 5t61 coloured by chain, front view.
PDB entry 5t61 coloured by chain, side view.
PDB entry 5t61 coloured by chain, top view.
Source organisms:
Primary publication:
The methanogenic CO2 reducing-and-fixing enzyme is bifunctional and contains 46 [4Fe-4S] clusters.
Wagner T, Ermler U, Shima S
Science 354 114-117 (2016)
PMID: 27846502

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166100 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Tungsten formylmethanofuran dehydrogenase subunit fwdA Chains: A, G, M, S, Y, e, k, q
Molecule details ›
Chains: A, G, M, S, Y, e, k, q
Length: 569 amino acids
Theoretical weight: 62.81 KDa
Source organism: Methanothermobacter wolfeii
Tungsten formylmethanofuran dehydrogenase subunit B Chains: B, H, N, T, Z, f, l, r
Molecule details ›
Chains: B, H, N, T, Z, f, l, r
Length: 432 amino acids
Theoretical weight: 48.41 KDa
Source organism: Methanothermobacter sp. CaT2
Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C Chains: C, I, O, U, a, g, m, s
Molecule details ›
Chains: C, I, O, U, a, g, m, s
Length: 270 amino acids
Theoretical weight: 28.61 KDa
Source organism: Methanothermobacter wolfeii
Tungsten formylmethanofuran dehydrogenase subunit fwdD Chains: D, J, P, V, b, h, n, t
Molecule details ›
Chains: D, J, P, V, b, h, n, t
Length: 130 amino acids
Theoretical weight: 14.41 KDa
Source organism: Methanothermobacter wolfeii
Tungsten formylmethanofuran dehydrogenase subunit fwdG Chains: E, K, Q, W, c, i, o, u
Molecule details ›
Chains: E, K, Q, W, c, i, o, u
Length: 82 amino acids
Theoretical weight: 8.56 KDa
Source organism: Methanothermobacter wolfeii
Tungsten formylmethanofuran dehydrogenase subunit fwdF Chains: F, L, R, X, d, j, p, v
Molecule details ›
Chains: F, L, R, X, d, j, p, v
Length: 349 amino acids
Theoretical weight: 38.62 KDa
Source organism: Methanothermobacter wolfeii

Ligands and Environments

10 bound ligands:
Ligand ZN 16 x ZN
Ligand MFN 8 x MFN
Ligand NA 7 x NA
Ligand K 57 x K
Ligand SF4 92 x SF4
Ligand W 8 x W
Ligand MGD 16 x MGD
Ligand H2S 8 x H2S
Ligand MG 8 x MG
Ligand CL 1 x CL
1 modified residue:
Ligand KCX 8 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 174.183Å b: 173.681Å c: 183.162Å
α: 89.98° β: 95.42° γ: 92.14°
R-values:
R R work R free
0.231 0.229 0.259

Quick links

Citations

16 review citations

The growing tree of Archaea: new perspectives on their diversity, evolution and ecology.
Adam et al. (2017)
15 more

2 mentions without citation

Metals in Prebiotic Catalysis: A Possible Evolutionary Pathway for the Emergence of Metalloproteins.
Aithal et al. (2023)
1 more