› 5t7b

X-ray diffraction
2.53Å resolution

Argonaute-2 - 5'-(E)-vinylphosphonate 2'-O-methyl-uridine modified mrTTR guide RNA complex

Released:
DOI: 10.2210/pdb5t7b/pdb
PDB entry 5t7b coloured by chain, front view.
PDB entry 5t7b coloured by chain, side view.
PDB entry 5t7b coloured by chain, top view.
Source organisms:
Entry authors: Elkayam E, Joshua-Tor L

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193883 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein argonaute-2 Chain: A
Molecule details ›
Chain: A
Length: 861 amino acids
Theoretical weight: 97.48 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UKV8 (Residues: 1-859; Coverage: 100%)
Gene names: AGO2, EIF2C2
Sequence domains:
Structure domains:
RNA (UVP)UAUAGAGCAAGAACACUGUU Chain: R
Molecule details ›
Chain: R
Length: 21 nucleotides
Theoretical weight: 6.73 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand IPH 2 x IPH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 63.207Å b: 108.306Å c: 68.821Å
α: 90° β: 106.62° γ: 90°
R-values:
R R work R free
0.196 0.194 0.238
Expression systems:
  • Spodoptera frugiperda
  • Not provided

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