› 5tgt

X-ray diffraction
2.45Å resolution

Crystal structure of glytamyl-tRNA synthetase GluRS from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5tgt/pdb
PDB entry 5tgt coloured by chain, front view.
PDB entry 5tgt coloured by chain, side view.
PDB entry 5tgt coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194992 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 502 amino acids
Theoretical weight: 57.86 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9XCL6 (Residues: 1-494; Coverage: 100%)
Gene names: PA3134, gltX
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 2PE 1 x 2PE
Ligand SO4 13 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 46.84Å b: 138.66Å c: 202.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links