› 5ti8

X-ray diffraction
2.07Å resolution

Crystal Structure of an aspartate aminotransferase from Pseudomonas

Released:
DOI: 10.2210/pdb5ti8/pdb
PDB entry 5ti8 coloured by chain, front view.
PDB entry 5ti8 coloured by chain, side view.
PDB entry 5ti8 coloured by chain, top view.
Source organism: Pseudomonas sp. M1
Primary publication:
Crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC.
Wilding M, Scott C, Newman J, Peat TS
Acta Crystallogr F Struct Biol Commun 73 29-35 (2017)
PMID: 28045391

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 474 amino acids
Theoretical weight: 52.42 KDa
Source organism: Pseudomonas sp. M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: W5IS25 (Residues: 1-454; Coverage: 100%)
Gene name: PM1_0202430
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Unit cell:
a: 76.444Å b: 95.429Å c: 114.449Å
α: 90° β: 100.21° γ: 90°
R-values:
R R work R free
0.187 0.184 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Pathway Driven Target Selection in Klebsiella pneumoniae: Insights Into Carbapenem Exposure.
Serral et al. (2022)

4 mentions without citation

Strategic single point mutation yields a solvent- and salt-stable transaminase from Virgibacillus sp. in soluble form.
Guidi et al. (2018)
3 more