› 5tja

X-ray diffraction
2.3Å resolution

I-II linker of TRPML1 channel at pH 6

Released:
DOI: 10.2210/pdb5tja/pdb
PDB entry 5tja coloured by chain, front view.
PDB entry 5tja coloured by chain, side view.
PDB entry 5tja coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of dual Ca2+/pH regulation of the endolysosomal TRPML1 channel.
Li M, Zhang WK, Benvin NM, Zhou X, Su D, Li H, Wang S, Michailidis IE, Tong L, Li X, Yang J
Nat Struct Mol Biol 24 205-213 (2017)
PMID: 28112729

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-190453 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mucolipin-1 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 25.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZU1 (Residues: 84-296; Coverage: 37%)
Gene names: MCOLN1, ML4, MSTP080, TRPML1
Sequence domains: Mucolipin, extracytosolic domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: I422
Unit cell:
a: 125.321Å b: 125.321Å c: 76.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.253
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Lysosomal Ca2+ Homeostasis and Signaling in Health and Disease.
Lloyd-Evans et al. (2020)
23 more

6 mentions without citation

Structural basis of dual Ca2+/pH regulation of the endolysosomal TRPML1 channel.
Li et al. (2017)
5 more