› 5tjb

X-ray diffraction
2.4Å resolution

I-II linker of TRPML1 channel at pH 4.5

Released:
DOI: 10.2210/pdb5tjb/pdb
PDB entry 5tjb coloured by chain, front view.
PDB entry 5tjb coloured by chain, side view.
PDB entry 5tjb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of dual Ca2+/pH regulation of the endolysosomal TRPML1 channel.
Li M, Zhang WK, Benvin NM, Zhou X, Su D, Li H, Wang S, Michailidis IE, Tong L, Li X, Yang J
Nat Struct Mol Biol 24 205-213 (2017)
PMID: 28112729

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190453 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mucolipin-1 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 25.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZU1 (Residues: 84-296; Coverage: 37%)
Gene names: MCOLN1, ML4, MSTP080, TRPML1
Sequence domains: Mucolipin, extracytosolic domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P4212
Unit cell:
a: 94.353Å b: 94.353Å c: 50.715Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.252
Expression system: Escherichia coli

Quick links

Citations

25 review citations

TRPML1: The Ca(2+)retaker of the lysosome.
Di Paola et al. (2018)
24 more

2 mentions without citation

Structural basis of dual Ca2+/pH regulation of the endolysosomal TRPML1 channel.
Li et al. (2017)
1 more