› 5tkv

X-ray diffraction
2.7Å resolution

X-RAY CRYSTAL STRUCTURE OF THE "CLOSED" CONFORMATION OF CTP-INHIBITED E. COLI CYTIDINE TRIPHOSPHATE (CTP) SYNTHETASE

Released:
DOI: 10.2210/pdb5tkv/pdb
PDB entry 5tkv coloured by chain, front view.
PDB entry 5tkv coloured by chain, side view.
PDB entry 5tkv coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Human CTP synthase filament structure reveals the active enzyme conformation.
Lynch EM, Hicks DR, Shepherd M, Endrizzi JA, Maker A, Hansen JM, Barry RM, Gitai Z, Baldwin EP, Kollman JM
Nat Struct Mol Biol 24 507-514 (2017)
PMID: 28459447

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141591 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTP synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 565 amino acids
Theoretical weight: 62.59 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A7E5 (Residues: 1-545; Coverage: 100%)
Gene names: JW2751, b2780, pyrG
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand GLN 2 x GLN
Ligand SO4 8 x SO4
Ligand MPD 2 x MPD
Ligand MG 2 x MG
Ligand CTP 2 x CTP
Ligand MRD 2 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P21212
Unit cell:
a: 159.159Å b: 110.675Å c: 129.487Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.157 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Cellular sensing by phase separation: Using the process, not just the products.
Yoo et al. (2019)
15 more

2 mentions without citation

Human CTP synthase filament structure reveals the active enzyme conformation.
Lynch et al. (2017)
1 more