› 5tlk

X-ray diffraction
2.7Å resolution

COMPLEX BETWEEN HUMAN CD27 AND FAB FRAGMENTS OF ANTIBODIES M2177 AND H2191

Released:
DOI: 10.2210/pdb5tlk/pdb
PDB entry 5tlk coloured by chain, front view.
PDB entry 5tlk coloured by chain, side view.
PDB entry 5tlk coloured by chain, top view.
Source organisms:
Primary publication:
Epitope-dependent mechanisms of CD27 neutralization revealed by X-ray crystallography.
Obmolova G, Teplyakov A, Malia TJ, Wunderler N, Kwok D, Barone L, Sweet R, Ort T, Scully M, Gilliland GL
Mol Immunol 83 92-99 (2017)
PMID: 28119207

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230912 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
M2177 LIGHT CHAIN Chains: A, E
Molecule details ›
Chains: A, E
Length: 218 amino acids
Theoretical weight: 23.71 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
M2177 HEAVY CHAIN Chains: B, F
Molecule details ›
Chains: B, F
Length: 229 amino acids
Theoretical weight: 24.44 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
H2191 LIGHT CHAIN Chains: C, G
Molecule details ›
Chains: C, G
Length: 218 amino acids
Theoretical weight: 24.07 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
H2191 HEAVY CHAIN Chains: D, H
Molecule details ›
Chains: D, H
Length: 226 amino acids
Theoretical weight: 24.12 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
CD27 antigen Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 109 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P26842 (Residues: 21-121; Coverage: 42%)
Gene names: CD27, TNFRSF7
Sequence domains: TNFR/NGFR cysteine-rich region

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
1 modified residue:
Ligand PCA 4 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 139.71Å b: 52.96Å c: 140.6Å
α: 90° β: 109.52° γ: 90°
R-values:
R R work R free
0.214 0.213 0.263
Expression systems:
  • Homo sapiens
  • unidentified baculovirus

Quick links

Citations

6 mentions without citation

SEPPA 3.0-enhanced spatial epitope prediction enabling glycoprotein antigens.
Zhou et al. (2019)
5 more