› 5tsq

X-ray diffraction
1.53Å resolution

Crystal structure of IUnH from Leishmania braziliensis in complex with D-Ribose

Released:
DOI: 10.2210/pdb5tsq/pdb
PDB entry 5tsq coloured by chain, front view.
PDB entry 5tsq coloured by chain, side view.
PDB entry 5tsq coloured by chain, top view.
Source organism: Leishmania braziliensis braziliensis
Entry authors: Bachega JFR, Timmers LFSM, Dalberto PF, Martinelli L, Pinto AFM, Basso LA, Norberto de Souza O, Santos DS

Function and Biology Details

Reaction catalysed: 
A purine nucleoside + H(2)O = D-ribose + a purine base
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107309 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine/uridine-preferring nucleoside hydrolase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 34.25 KDa
Source organism: Leishmania braziliensis braziliensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A4H9Q9 (Residues: 2-313; Coverage: 100%)
Gene name: LBRM_18_1610
Sequence domains: Inosine-uridine preferring nucleoside hydrolase
Structure domains: Ribonucleoside hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand BDR 1 x BDR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: I222
Unit cell:
a: 74.66Å b: 94.08Å c: 123.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.168
Expression system: Escherichia coli

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