› 5tsw

X-ray diffraction
2.5Å resolution

HIGH RESOLUTION CRYSTAL STRUCTURE OF A HUMAN TNF-ALPHA MUTANT

Released:
DOI: 10.2210/pdb5tsw/pdb
PDB entry 5tsw coloured by chain, front view.
PDB entry 5tsw coloured by chain, side view.
PDB entry 5tsw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High resolution crystal structure of a human tumor necrosis factor-alpha mutant with low systemic toxicity.
Cha SS, Kim JS, Cho HS, Shin NK, Jeong W, Shin HC, Kim YJ, Hahn JH, Oh BH
J Biol Chem 273 2153-60 (1998)
PMID: 9442056

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134285 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tumor necrosis factor, soluble form Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 150 amino acids
Theoretical weight: 16.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01375 (Residues: 84-233; Coverage: 64%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE M18X
Spacegroup: P212121
Unit cell:
a: 94.17Å b: 94.56Å c: 95.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.299
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

TNF alpha and the TNF receptor superfamily: structure-function relationship(s).
Idriss et al. (2000)
1 more

1 mention without citation

Crystallization and preliminary X-ray analysis of the tumour necrosis factor alpha-tumour necrosis factor receptor type 2 complex.
Mukai et al. (2009)