› 5tws

X-ray diffraction
1.85Å resolution

Post-catalytic complex of human Polymerase Mu (H329A) with newly incorporated UTP

Released:
DOI: 10.2210/pdb5tws/pdb
PDB entry 5tws coloured by chain, front view.
PDB entry 5tws coloured by chain, side view.
PDB entry 5tws coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural accommodation of ribonucleotide incorporation by the DNA repair enzyme polymerase Mu.
Moon AF, Pryor JM, Ramsden DA, Kunkel TA, Bebenek K, Pedersen LC
Nucleic Acids Res 45 9138-9148 (2017)
PMID: 28911097

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113190 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct DNA/RNA hybrid molecule
Macromolecules (4 distinct):
DNA-directed DNA/RNA polymerase mu Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 39.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NP87 (Residues: 134-397, 411-494; Coverage: 70%)
Gene names: POLM, polmu
Sequence domains:
Structure domains:
DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3') Chain: T
Molecule details ›
Chain: T
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
DNA (5'-D(P*GP*CP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 4 nucleotides
Theoretical weight: 1.19 KDa
DNA/RNA (5'-D(*CP*GP*TP*A)-R(P*U)-3') Chain: P
Molecule details ›
Chain: P
Length: 5 nucleotides
Theoretical weight: 1.5 KDa

Ligands and Environments

6 bound ligands:
Ligand MG 1 x MG
Ligand NA 2 x NA
Ligand GOA 1 x GOA
Ligand CL 4 x CL
Ligand EDO 1 x EDO
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 59.935Å b: 68.858Å c: 110.319Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.2
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Ribonucleotide discrimination by translesion synthesis DNA polymerases.
Vaisman et al. (2018)
4 more