› 5udl

X-ray diffraction
1.65Å resolution

IFIT1 N216A monomeric mutant (L457E/L464E) with m7Gppp-AAAA (anti conformation of cap)

Released:
DOI: 10.2210/pdb5udl/pdb
PDB entry 5udl coloured by chain, front view.
PDB entry 5udl coloured by chain, side view.
PDB entry 5udl coloured by chain, top view.
Source organisms:
Primary publication:
Structure of human IFIT1 with capped RNA reveals adaptable mRNA binding and mechanisms for sensing N1 and N2 ribose 2'-O methylations.
Abbas YM, Laudenbach BT, Martínez-Montero S, Cencic R, Habjan M, Pichlmair A, Damha MJ, Pelletier J, Nagar B
Proc Natl Acad Sci U S A 114 E2106-E2115 (2017)
PMID: 28251928

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140811 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Interferon-induced protein with tetratricopeptide repeats 1 Chain: A
Molecule details ›
Chain: A
Length: 479 amino acids
Theoretical weight: 55.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09914 (Residues: 1-478; Coverage: 100%)
Gene names: G10P1, IFI56, IFIT1, IFNAI1, ISG56
Sequence domains:
RNA (5'-D(*(GTA))-R(P*AP*AP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 4 nucleotides
Theoretical weight: 1.71 KDa
Source organism: unidentified
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand PGE 1 x PGE
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P4222
Unit cell:
a: 111.76Å b: 111.76Å c: 92.992Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.182
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

30 review citations

Biochemistry and Molecular Biology of Flaviviruses.
Barrows et al. (2018)
29 more

4 mentions without citation

MultiBac: from protein complex structures to synthetic viral nanosystems.
Pelosse et al. (2017)
3 more