› 5ufh

X-ray diffraction
1.45Å resolution

The crystal structure of a LacI-type transcription regulator from Bifidobacterium animalis subsp. lactis DSM 10140

Released:
DOI: 10.2210/pdb5ufh/pdb
PDB entry 5ufh coloured by chain, front view.
PDB entry 5ufh coloured by chain, side view.
PDB entry 5ufh coloured by chain, top view.
Source organism: Bifidobacterium animalis subsp. lactis AD011
Entry authors: Tan K, Li H, Endres M, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lacI-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 347 amino acids
Theoretical weight: 38.72 KDa
Source organism: Bifidobacterium animalis subsp. lactis AD011
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8DV56 (Residues: 2-344; Coverage: 100%)
Gene name: BLA_0054
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand NO3 22 x NO3
2 modified residues:
Ligand MSE 15 x MSE
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 72.326Å b: 60.171Å c: 67.188Å
α: 90° β: 109.22° γ: 90°
R-values:
R R work R free
0.166 0.165 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links