› 5ugw

X-ray diffraction
2.31Å resolution

STRUCTURE OF THE HUMAN TELOMERASE THUMB DOMAIN

Released:
DOI: 10.2210/pdb5ugw/pdb
PDB entry 5ugw coloured by chain, front view.
PDB entry 5ugw coloured by chain, side view.
PDB entry 5ugw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Analysis Reveals the Deleterious Effects of Telomerase Mutations in Bone Marrow Failure Syndromes.
Hoffman H, Rice C, Skordalakes E
J Biol Chem 292 4593-4601 (2017)
PMID: 28154186

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127089 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Telomerase reverse transcriptase Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14746 (Residues: 961-1132; Coverage: 15%)
Gene names: EST2, TCS1, TERT, TRT
Sequence domains: Telomerase reverse transcriptase, C-terminal extension

Ligands and Environments

1 bound ligand:
Ligand GSH 2 x GSH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P321
Unit cell:
a: 92.425Å b: 92.425Å c: 50.178Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.197 0.209
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Molecular mechanisms of telomere biology disorders.
Grill et al. (2021)
2 more

11 mentions without citation

Structural biology of telomeres and telomerase.
Smith et al. (2020)
10 more