› 5uj7

X-ray diffraction
3.39Å resolution

Structure of the active form of human Origin Recognition Complex ATPase motor module, complex subunitS 1, 4, 5

Released:
DOI: 10.2210/pdb5uj7/pdb
PDB entry 5uj7 coloured by chain, front view.
PDB entry 5uj7 coloured by chain, side view.
PDB entry 5uj7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the active form of human origin recognition complex and its ATPase motor module.
Tocilj A, On KF, Yuan Z, Sun J, Elkayam E, Li H, Stillman B, Joshua-Tor L
Elife 6 (2017)
PMID: 28112645

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129159 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Origin recognition complex subunit 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 44.18 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: Q13415 (Residues: 471-861; Coverage: 45%)
Gene names: ORC1, ORC1L, PARC1
Sequence domains:
Origin recognition complex subunit 4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 436 amino acids
Theoretical weight: 50.44 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: O43929 (Residues: 1-436; Coverage: 100%)
Gene names: ORC4, ORC4L
Sequence domains:
Origin recognition complex subunit 5 Chains: E, F
Molecule details ›
Chains: E, F
Length: 284 amino acids
Theoretical weight: 33.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: O43913 (Residues: 1-284; Coverage: 65%)
Gene names: ORC5, ORC5L
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 6 x ATP
Ligand MG 4 x MG
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 120.887Å b: 81.141Å c: 151.947Å
α: 90° β: 97.25° γ: 90°
R-values:
R R work R free
0.245 0.242 0.281
Expression system: Spodoptera aff. frugiperda 2 RZ-2014

Quick links

Citations

5 review citations

From structure to mechanism-understanding initiation of DNA replication.
Riera et al. (2017)
4 more

4 mentions without citation

An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells.
Vinogradova et al. (2020)
3 more