› 5una

X-ray diffraction
2.55Å resolution

Fragment of 7SK snRNA methylphosphate capping enzyme

Released:
DOI: 10.2210/pdb5una/pdb
PDB entry 5una coloured by chain, front view.
PDB entry 5una coloured by chain, side view.
PDB entry 5una coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Wu H, Tempel W, Dombrovski L, McCarthy AA, Loppnau P, Bountra C, Arrowsmith CH, Edwards AM, Park H, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181627 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
7SK snRNA methylphosphate capping enzyme Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 292 amino acids
Theoretical weight: 33.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7L2J0 (Residues: 400-689; Coverage: 42%)
Gene names: BCDIN3, MEPCE
Sequence domains: Bicoid-interacting protein 3 (Bin3)
Structure domains: Vaccinia Virus protein VP39
unidentified peptide section/fragment Chains: M, N, O, Q, R
Molecule details ›
Chains: M, N, O, Q, R
Length: 6 amino acids
Theoretical weight: 529 Da
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand SAH 6 x SAH
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: R3
Unit cell:
a: 183.24Å b: 183.24Å c: 151.39Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.217
Expression system: Escherichia coli

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