› 5uoh

X-ray diffraction
2.61Å resolution

Crystal Structure of Hip1 (Rv2224c) T466A mutant

Released:
DOI: 10.2210/pdb5uoh/pdb
PDB entry 5uoh coloured by chain, front view.
PDB entry 5uoh coloured by chain, side view.
PDB entry 5uoh coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structure Determination of Mycobacterium tuberculosis Serine Protease Hip1 (Rv2224c).
Naffin-Olivos JL, Daab A, White A, Goldfarb NE, Milne AC, Liu D, Baikovitz J, Dunn BM, Rengarajan J, Petsko GA, Ringe D
Biochemistry 56 2304-2314 (2017)
PMID: 28346784

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease Hip1 Chain: A
Molecule details ›
Chain: A
Length: 492 amino acids
Theoretical weight: 52.95 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WHR3 (Residues: 14-14, 35-520; Coverage: 93%)
Gene names: MTCY427.05c, Rv2224c, caeA, hip1
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P3121
Unit cell:
a: 104.249Å b: 104.249Å c: 127.707Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.217 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Chemical approaches to unraveling the biology of mycobacteria.
Finin et al. (2023)
4 other articles

2 mentions without citation

Structure Determination of Mycobacterium tuberculosis Serine Protease Hip1 (Rv2224c).
Naffin-Olivos et al. (2017)
1 more